Chemical SOPs and Guidelines

The Standard Operating Procedures (SOPs) and Guidelines describe safe handling for various chemical hazard classes and some commonly-used chemicals.

Customized, Laboratory-Specific SOPs

Chemicals identified as particularly hazardous or high risk require a customized, laboratory-specific SOP. For these chemicals:

Customized SOPs may reference appropriate chemical safety guidelines linked below.

* Means customization is required. Where a guideline is provided, use the SOP template to create a customized SOP.

Physical Hazards

Compressed Gases Revised 9/30/2016
Cryogens* Revised 8/17/2021
Explosives * Revised 10/8/2012
Flammable Liquids Revised 11/28/2016
Oxidizers Revised 10/17/2016
            Aqua Regia* Revised 10/17/2019
            Nitric Acid * Revised 7/25/2019 and Chemical Incompatibility Information
            Perchloric Acid *Revised 12/28/2017
            Piranha* Revised 10/17/2019
Organic Peroxide-Formers Revised 9/4/2019
Pressure and Vacuum Revised 2/26/2013
Pyrophoric and other highly reactive materials *Revised 8/3/2012
Water Reactives * Revised 3/8/2016

Health Hazards

Asphyxiants Revised 1/2013
Biologically-Derived Substances * Questions? Contact Biological Safety Division at biosafety@duke.edu.
Corrosives Revised 10/17/2016
            Aqua Regia* Revised 10/17/2019
            Bleach Revised 2/7/2017 and Chemical Incompatibility Information
            Hydrofluoric Acid * Revised 7/25/2019 and Hydrofluoric Acid First Aid and Medical Guidelines Revised 6/21/2019
            PMSF and HF Releasers New! Revised 3/1/2021
            Nitric Acid * Revised 7/25/2019 and Chemical Incompatibility Information
            Perchloric Acid *Revised 12/28/2017
            Piranha* Revised 10/17/2019
            Sulfuric Acid* Revised 7/25/2019

Toxic Gases Revised 5/1/2013
            Phosgene * Revised 6/4/2014
Toxic and Health Hazard Liquids New! Revised 3/17/2022
            Chloroform  Revised 7/7/2020
            Formalin, paraformaldehyde solutions, paraformaldehyde powder * Revised 5/4/2021
            Methylene Chloride/Dichloromethane * Revised 7/12/2013
            Phenol * Revised 7/7/2020 and HAZARD ALERT - PHENOL BURNS CAN BE FATAL*Revised 2/15/2021
            b-Mercaptoethanol * Revised 7/25/2019
Toxic and Health Hazard Powders (and suspensions/solutions).* New! Revised 4/14/2021 - Now with fill-able fields for lab-specific information.
            Acrylamide * Revised 8/2/2012
            Cyanide Salts * Revised 7/25/2019
            Ethidium Bromide * Revised 2/12/2019
            Nanomaterials * Revised 5/1/2013
            Sodium Azide * Revised 3/5/2015
            Osmium Tetroxide * Revised 7/25/2019

* Means customization is required